Optimization Of the Composition of The Nutrient Medium and Selection of Growth Conditions to Increase the Production of Phytohormones in The T. Harzianum Uzcf-55 Strain
Keywords:
Auxin (AU), gibberellin (GK), indole acetic acid (ISK)Abstract
It was found that Mandels medium was optimal for T. harzianum 55, and Capek medium was optimal for T. harzianum 54, T. asperellum 12. However, in order to increase the production of phytohormones by these strains, the composition of the medium was changed to enrich and reduce the cost of the medium. That is, Mandels and Capek mediums were prepared with 2% sucrose and 2% other carbon sources (molasses, cottonseed meal, crushed cotton leaves and corn extract) added, the strains were grown in them, and the production of GC and Indole complexes was studied. It was found that all three strains produced the most active GC and Indole complexes in mediums enriched with corn extract and molasses. In the control variant, it was found that in these nutrient media, the amount of indole compounds synthesized by T. harzianum 55 was 0.559 mg/ml and the amount of GC was 0.172 mg/ml, the amount of indole compounds synthesized by P. Canescens 54 was 0.418 mg/ml, the amount of GC was 0.189 mg/ml, and the amount of indole compounds synthesized by F. moniliforme 12 was 0.518 mg/ml and the amount of GC was 0.194 mg/ml.
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